These are the items that appeared in the Biosphere.
The Diffusion Beam is obtained by defeating a trio of FG-1000 robots on Samus' first trip to the Biosphere.
Samus will activate the Space Jump and the Screw Attack without authorization while chasing the unknown Galactic Federation trooper. It is activated upon discovering that the bridge to the entrance of the Bioweapon Research Center was retracted and the Terminal on her side was destroyed.
The Seeker Missile Launcher is obtained while returning from the Bioweapon Research Center. It is obtained by defeating the Rhedogian.
In Experiment Floor 3, a sliding platform exists along the left wall close to the exit door. Samus must ride the platform (taking care to kill Reos that attack) to its end and navigate a complex network of Morph Ball tunnels to reach the Energy Tank.
Energy Part 1Edit
From the King Kihunter's chamber in the Biological Experiment Floor, Samus will find the Energy Part in a tunnel straight ahead. She will find the entrance to the tunnel up the slope on the right and in the left wall.
Energy Part 2Edit
From the north exit of the large room with the pillar made of plants, Samus must use the Grapple Beam to reach the cliff in the next room. From there, she must Space Jump to the alcove above the exit door, and destroy a small crate to attain the Part.
Energy Part 3Edit
Energy Part 4Edit
While a longer route involving pipes from the Observation Room and then the Grapple Beam can lead to this Part, Samus can also simply go up to the northern exit of the room and Space Jump to the ledge with the Part.
Energy Part 5Edit
Samus must return to the top ledge of the Swamp Zone where she fought the first Groganch. From here, she must Space Jump around to reach a platform. A sensor is on the wall here, activating it will deploy three platforms from the wall on the right. Samus must scale them quickly as they retract after a time to reach the Part. The Shinespark and Space Jump can also be used in tandem, without activating the sensor.
This can only be obtained after Samus' return to the BOTTLE SHIP. Returning to the massive chamber leading to the now sealed off Biosphere Test Area, Samus will need to deploy a Power Bomb. After killing the Desbrachian that is summoned, Samus can go through the hatch it guarded to find the E-Recovery Tank.
Missile Tank 1Edit
After Samus' first encounter with Griptians, she will reach this Tank through a tunnel in the wall of the next corridor.
Missile Tank 2Edit
Missile Tank 3Edit
Missile Tank 4Edit
Immediately following the Ghalmanian elevator sequence, Samus will find a hidden tunnel at the top. The Tank is at the end.
Missile Tank 5Edit
In a corridor soon after the elevator shaft, Samus will find another tunnel in the wall leading to this Tank.
Missile Tank 6Edit
Through a tunnel in the large observation deck of the Observation Room. Getting this is risky, as Samus may slide down a slope through a hole and fail to get the Tank. The Spring Ball is effective at obtaining the Tank. If she falls, a well-timed Bomb Jump will bring her back up.
Missile Tank 7Edit
Missile Tank 8Edit
While ascending the Biological Experiment Floor, Samus will find the Tank on a ledge below the main spiral leading to the top.
Missile Tank 9Edit
Missile Tank 10Edit
Missile Tank 11Edit
This is in the large Bull-inhabited cavern with rising and falling water levels. The Tank is in a tunnel in the corner of the far end of the room.
Missile Tank 12Edit
Missile Tank 13Edit
In the same chamber as Energy Part 2, Samus will find this Tank at the top of the mountain she needs to pass. The Tank is hidden in a small crate in a camouflaged tunnel on the right of the cliff.
Missile Tank 14Edit
This item may not be possible to obtain until after Samus' return to the BOTTLE SHIP. In the large room where the Ghalmanians are first fought, Samus must latch onto a Grapple Point and pull out of the main chamber, where she will find the Tank in a small crate.
Missile Tank 15Edit
Missile Tank 16Edit
At the end of a long jungle corridor leading to the Biological Experiment Floor. Samus must disable a holographic generator to reveal the industrialized scenery, destroy a Super Missile obstacle and Space Jump to the opened alcove to reach the Tank.
Missile Tank 17Edit
Samus must Shinespark through a hole in the ceiling of the room before the Ghalmanian elevator (the Shinespark can be built up in the corridor behind it). Once she reaches the top, she must drop back down and grab a ledge halfway down the shaft on the left. The Tank is in a small crate.
Missile Tank 18Edit
In the same corridor as Missile Tank 5, behind a Super Missile blast shield on the left.
Missile Tank 19Edit
Back in the Breeding Room area, Samus must dismantle a Super Missile blast shield, and then Shinespark up the shaft that's revealed to find the Tank on a higher ledge.
Missile Tank 20Edit
Missile Tank 21Edit
From the bottom of the room where Samus encountered little birdie, Samus must run up the slippery slope. From there, she must slide back down and launch a Super Missile at a blast shield on the ceiling to reveal a Grapple Point. She must then run back up, once again slide down and grapple onto the point, which will bring her into a hidden chamber.
Missile Tank 22Edit
Missile Tank 23Edit
In the same submerged corridor as Missile Tank 10, Samus must run through the water with the Gravity Feature (shooting any Skultera in her way) and penetrate a metal grate guarding the Tank.
Missile Tank 24Edit
Through the Navigation Booth close to the first Groganch's room is an L-shaped hallway inhabited by Zeros and Kihunters. The Space Jump must be used to claim this Tank in an alcove above the exit around the corner.
Missile Tank 25Edit
In the observation area of the Groganch's room, Samus must go through the door on the right and move into the next corridor. When she spots a sensor, she must activate it so that part of the glass wall will open and give her access to the Tank.
Missile Tank 26Edit
The final Missile Tank in the Biosphere is situated on a high ledge in the room before the Biosphere Test Area. In the forest room where the second Groganch is encountered, Samus must speed run on the track through a wall in a cliff, and continue running until she conveniently reaches the Tank's ledge.
Through a tunnel where Groganches can be seen, Samus will come to a ruined lounge. She must go into the ladies' restroom from here, and she will find the Charge in the far-left stall.