|This article is written from the Real Life point of view|
In Echoes, the theme is heard during various points whenever Dark Samus is present and/or nearby. It is also heard when Dark Samus attempts to steal Samus's Light Suit after being defeated in the Sky Temple Grounds at the end of the game.
Corruption features the track in various scenes involving Dark Samus, including her resurrection within the Colossus at the beginning of the game, before she ambushes Samus, Rundas, Ghor and Gandrayda on Norion, and each time she absorbs one of the latter three Hunters, after Samus defeats them. Its final appearance occurs when Dark Samus is about to fight Samus herself on Phaaze.
The theme can be heard here: 
- ^ In-game files. The track's actual filename is "darksamus-mae", but every usage of the theme is named "Dark Samus Intro". "Mae" is Japanese for "before".
|Metroid Prime and Dark Samus|
|Abilities|| Metroid Prime: Particle Wave Projector • Seeker Missile • Snare Beam • Ultrafrigid Beam|
Dark Samus: Boost Ball • Charge Beam • Dark Echo • Missile Launcher • Phazon bullets • Shrapnel Beam • Stealth field generator • Varia Suit
|Misc.||VS Metroid Prime (Spider) • Metroid Prime Battle • Dark Samus Intro • Darkness • Escape • Dark Samus Biography • Dark Samus Gallery • Dark Samus Rooms|