Biosphere/Items

These are the items that appeared in the Biosphere.

Major Items

Diffusion Beam

Diffusion Beam
Other Requirements	Destroy three FG-1000s

The Diffusion Beam is obtained by defeating a trio of FG-1000 robots on Samus' first trip to the Biosphere.

Space Jump/Screw Attack

Space Jump/Screw Attack
Required items	Plasma Beam

Samus will activate the Space Jump and the Screw Attack without authorization while chasing the unknown Galactic Federation trooper. It is activated upon discovering that the bridge to the entrance of the Bioweapon Research Center was retracted and the Terminal on her side was destroyed.

Seeker Missile Launcher

Seeker Missile Launcher
Required items	Space Jump/Screw Attack
Other Requirements	Return from the Bioweapon Research Center, defeat the Rhedogian

The Seeker Missile Launcher is obtained while returning from the Bioweapon Research Center. It is obtained by defeating the Rhedogian in the "confrontation room".

Energy Tank

Energy Tank
Required items	none
Other Requirements	Defeat Groganch

In Experiment Floor 3, a sliding platform exists along the left wall close to the exit door. Samus must ride the platform (taking care to kill Reos that attack) to its end and navigate a complex network of Morph Ball tunnels to reach the Energy Tank.

Energy Parts

Energy Part 1

Energy Part 1
Required items	Morph Ball
Other Requirements	Kill King Kihunter

From the King Kihunter's chamber in the Biological Experiment Floor, Samus will find the Energy Part in a tunnel straight ahead. She will find the entrance to the tunnel up the slope on the right and in the left wall.

Energy Part 2

Energy Part 2
Required items	Grapple Beam, Space Jump/Screw Attack

From the north exit of the "grand tree room", Samus must use the Grapple Beam to reach the cliff in the "jungle hallway". From there, she must Space Jump to the alcove above the exit door, and destroy a small crate to attain the Part.

Energy Part 3

Energy Part 3
Required items	Power Bombs

In one of the corners of the Subterranean Control Room is a Desbrachian shield. After killing it, the Energy Part can be found in the chamber it was guarding.

Energy Part 4

Energy Part 4
Required items	Grapple Beam/Space Jump

While a longer route involving pipes from the Observation Room and then the Grapple Beam can lead to this Part, Samus can also simply go up to the northern exit of the room and Space Jump to the ledge with the Part.

Energy Part 5

Energy Part 5
Required items	Space Jump

Samus must return to the top ledge of the Swamp Zone where she fought the first Groganch. From here, she must Space Jump around to reach a platform. A sensor is on the wall here, activating it will deploy three platforms from the wall on the right. Samus must scale them quickly as they retract after a time to reach the Part. The Shinespark and Space Jump can also be used in tandem, without activating the sensor.

E-Recovery Tank

E-Recovery Tank
Required items	Power Bombs
Other Requirements	Kill a Desbrachian

This can only be obtained after Samus' return to the BOTTLE SHIP. Returning to the massive catwalk leading to the now sealed off Biosphere Test Area, Samus will need to deploy a Power Bomb. After killing the Desbrachian that is summoned, Samus can go through the hatch it guarded to find the E-Recovery Tank.

Missile Tanks

Missile Tank 1

Missile Tank 1
Required items	none

After Samus's first encounter with Griptians in the rainforest, she will reach this Tank through a tunnel in the wall of the next corridor.

Missile Tank 2

Missile Tank 2
Required items	none
Other Requirements	Disable holographic generator

In the long pathway, Samus must power down the holographic generator to reveal this Tank on a metallic platform.

Missile Tank 3

Missile Tank 3
Required items	Morph Ball

Beyond the Observation Room is an unimportant "Geemer-infested corridor". After clearing them out, either by triggering a sensor to gas them or killing them outright, the Tank can be found in a small chamber inside the wall.

Missile Tank 4

Missile Tank 4
Required items	Morph Ball

Immediately following the sequence in the "Ghalmanian shaft", Samus will find a hidden tunnel at the top. The Tank is at the end.

Missile Tank 5

Missile Tank 5
Required items	none

In an "illuminated corridor" soon after the elevator shaft, Samus will find another tunnel in the wall leading to this Tank.

Missile Tank 6

Missile Tank 6
Required items	none

Through a tunnel in the large observation deck of the Observation Room. Getting this is risky, as Samus may slide down a slope through a hole and fail to get the Tank. The Spring Ball is effective at obtaining the Tank. If she falls, a well-timed Bomb Jump will bring her back up.

Missile Tank 7

Missile Tank 7
Required items	Missiles

Clearing the rubbish in the Scrap Block will reveal a tunnel into the wall of the adjacent long dirt tunnel, through which this Tank is accessible.

Missile Tank 8

Missile Tank 8
Required items	none

While ascending the Biological Experiment Floor, Samus will find the Tank on a ledge below the main spiral leading to the top.

Missile Tank 9

Missile Tank 9
Required items	none

In the room where Samus self-authorizes the Space Jump and Screw Attack, she can fall down into the pit and roll through a tunnel on the left to reach this Tank.

Missile Tank 10

Missile Tank 10
Required items	Missiles

Samus must plunge into the water-filled tank and kill a Whipvine to unblock the tunnel with this Tank.

Missile Tank 11

Missile Tank 11
Required items	none

This is in the large water-filled room with rising and falling water levels. The Tank is in a tunnel in the corner of the far end of the room.

Missile Tank 12

Missile Tank 12
Required items	none

In a damaged elevator shaft inhabited by Geemers (close to the first Groganch's room). Samus must use Search View to find a small crate on the ceiling and destroy the crate to obtain the Tank.

Missile Tank 13

Missile Tank 13
Required items	Grapple Beam, Bombs

In the same room as Energy Part 2, Samus will find this Tank at the top of the mountain she needs to pass. The Tank is hidden in a small crate in a camouflaged tunnel on the right of the cliff.

Missile Tank 14

Missile Tank 14
Required items	Grapple Beam

This item may not be possible to obtain until after Samus's return to the BOTTLE SHIP. In the "confrontation room" where the Ghalmanians are first fought, Samus must latch onto a Grapple Point and pull out of the main chamber, where she will find the Tank in a small crate.

Missile Tank 15

Missile Tank 15
Required items	Speed Booster

In the long dirt tunnel leading to the Subterranean Control Room, Samus must Shinespark up a shaft and then drop down onto a ledge, where the Tank is in a small crate.

Missile Tank 16

Missile Tank 16
Required items	Super Missiles, Space Jump
Other Requirements	Disable Holographic Generator

At the end of the jungle environment. Samus must disable a holographic generator to reveal the industrialized scenery, destroy a Super Missile obstacle and Space Jump to the opened alcove to reach the Tank.

Missile Tank 17

Missile Tank 17
Required items	Speed Booster, Bombs

Samus must Shinespark through a hole in the ceiling of the lower "Ghalmanian shaft", outside the elevator shaft (the Shinespark can be built up in the corridor behind it). Once she reaches the top, she must drop back down and grab a ledge halfway down the shaft on the left. The Tank is in a small crate.

Missile Tank 18

Missile Tank 18
Required items	Super Missiles

In the same corridor as Missile Tank 5, behind a Super Missile blast shield on the left.

Missile Tank 19

Missile Tank 19
Required items	Super Missiles, Speed Boosters

Back in the Breeding Room area, Samus must dismantle a Super Missile blast shield in the "double-sided corridor", and then Shinespark up the shaft in the adjacent stairwell to find the Tank on a higher ledge.

Missile Tank 20

Missile Tank 20
Required items	none

A Blast Shield in a "multi-door hallway" will open after Samus's return to the BOTTLE SHIP. Kick Climbing up a shaft will get Samus to this Tank underneath an elaborate tree.

Missile Tank 21

Missile Tank 21
Required items	Grapple Beam, Super Missiles, Speed Booster

From the bottom of the "rainforest" where Samus encountered little birdie, Samus must run up the slippery slope. From there, she must slide back down and launch a Super Missile at a blast shield on the ceiling to reveal a Grapple Point. She must then run back up, once again slide down and grapple onto the point, which will bring her into a hidden chamber.

Missile Tank 22

Missile Tank 22
Required items	Speed Booster

Samus must speed run up the spiraling walkway in the "grand tree room", and Shinespark at the dead end of the spiral to reach the Tank.

Missile Tank 23

Missile Tank 23
Required items	Speed Booster, Gravity Feature

In the same submerged corridor as Missile Tank 10, Samus must run through the water with the Gravity Feature (shooting any Skultera in her way) and penetrate a metal grate guarding the Tank.

Missile Tank 24

Missile Tank 24
Required items	Space Jump

Through the Navigation Booth close to the first Groganch's room is an L-shaped hallway inhabited by Zeros and Kihunters. The Space Jump must be used to claim this Tank in an alcove above the exit around the corner.

Missile Tank 25

Missile Tank 25
Required items	Wave Beam

In the observation area of the Swamp Zone, Samus must go through the door on the right and move into the next corridor. When she spots a sensor, she must activate it so that part of the glass wall will open and give her access to the Tank.

Missile Tank 26

Missile Tank 26
Required items	Speed Booster

The final Missile Tank in the Biosphere is situated on a high ledge in the room before the Biosphere Test Area. In Experiment Floor 3, Samus must speed run on the track through a wall in a cliff, and continue running until she conveniently reaches the Tank's ledge.

Accel Charge

Accel Charge
Required items	none

Through a tunnel where Groganches can be seen, Samus will come to a ruined lounge. She must go into the ladies' restroom from here, and she will find the Charge in the far-left stall.