In this room, there isn't an incubation tank like there is in Incubation Vault 01 and Incubation Vault 03. Instead, there is a walkway that Samus on the top housing two portals. When Samus first enters this room before getting to the boss, she will have to destroy the Crash Pillar, Electro Voldrums, Guardians, and Psycho Bits. Samus will later obtain the Shock Coil in this room.
- Shock Coil
- Samus must drop down through the side passage when entering from Incubation Vault 01 to fall next to the Shock Coil.
- ""TELEPORTATION DEVICE ONLINE. STEP INTO THE PORTAL TO WARP TO ANOTHER LOCATION."
- Blue Force Field
- "ANALYSIS INDICATES THE FORCE FIELD IS IMPERVIOUS TO MOST WEAPONS. A CONCENTRATED BLAST OF NEUTRINOS MAY DAMAGE ITS GUARD NODE."