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The Metroid Prime Theme is, as the name implies, the main theme of Metroid Prime. It is also featured in the Opening/Menu (Metroid Prime) song in Super Smash Bros. Brawl, though a narration by Jay Ward was added in the part where the opening theme is heard. The overall beat was similar to that of the main theme music for Metroid, although it included crackling and squeaking noises. While being its own song, it incorporates the iconic notes from the Title theme of the original Metroid.
The theme is present in Metroid Prime 2: Echoes Bonus Disc, where it is used in The Metroid Chronicle while playing a movie with clips of Prime. Metroid Prime Trilogy also features the theme as one song that can be purchased in the Soundtrack Gallery. Part of the theme plays on the Title Screen and Samus Data Screen of Metroid Fusion.