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Opening/Menu (Metroid Prime) is a theme in Super Smash Bros. Brawl playing on the Frigate Orpheon stage that consists of a medley of the Metroid Prime Theme from Metroid Prime and the Credits as well. A narration by Jay Ward, the voice actor for the characters Meta Knight and Wolf O'Donnell, was added to the opening title portion of the song, much like Main Theme (Metroid). The narration reads:
- "In the reaches of space, below the surface of planet Zebes, Samus Aran faced the Space Pirates. She destroyed their operation, wiped out the parasites called "Metroids", and defeated Mother Brain. But the Pirates were far from finished."
The theme can be heard here: .
Interestingly, some of this dialogue is from an unused female voice in Prime. 
The unused dialogue is as follows. Any dialogue reused in Brawl is in bold.:
- "Ten years ago, below the surface of Planet Zebes, the mercenaries known as Space Pirates were defeated by interstellar bounty hunter Samus Aran. Descending to the very core of the Pirate stronghold, Samus exterminated the energy-based parasites called Metroids, and defeated Mother Brain, the leader of the Pirate Horde. But the Space Pirates were far from finished. Several Pirate research vessels were orbiting Zebes while Samus fought on the surface below. After the fall of Mother Brain, the ships escaped, with the hopes of finding enough resources to rebuild their forces, and take their revenge. After discovering a possible Pirate colony on planet Tallon IV, Samus has again prepared for war, hoping to end the Pirate threat forever."