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Samus Aran's Appearance Fanfare plays every time Samus Aran starts the game or respawns from her gunship or a Save Station. It appears in all Metroid games virtually unchanged, apart from Metroid II: Return of Samus which uses a different tune. It is first heard just seconds before gameplay starts in the original Metroid.
In AR Games, the Prime variation of the theme plays when Samus' AR Card is scanned despite the fact that her model is from Other M.
- In Metroid Prime 3: Corruption, the theme does not play when starting a game or respawning unlike in Prime or Echoes. It does, however, play when Samus lands on a location.